Background: Mycobacterium tuberculosis, the causative agent of pulmonary tuberculosis, spreads via
droplets. The TNF-α-308 G/A gene polymorphism is one of the host genetic variables that may affect an
individual's vulnerability to the disease. However, this polymorphism has not been studied in Cirebon.
Aims: To analyze the TNF-α -308 G/A gene polymorphism as a risk factor for the occurrence of
pulmonary tuberculosis in Cirebon.
Methods: A total of 64 participants joined part in an analytical observational study using a case-control
design at the Biomolecular and Genetics Laboratory, Faculty of Medicine, Universitas Swadaya Gunung
Jati, Indonesia. DNA extraction from blood samples, ARMS-PCR genotyping, and 1.5% electrophoresis gel
visualization were all part of the data gathering process. The chi-square test was used to analyze the
data. This study including inclusion criteria, exclusion criteria, and sample control for the research.
Results: According to the study, there is no link between Cirebon's risk of pulmonary tuberculosis and
the polymorphism in the TNF-α-308 G/A gene (OR = 0.462; P > 0.05). However, the study shown a
protective factors which means that individuals with the TNF-α -308 G/A gene polymorphism have a
lower risk of developing pulmonary tuberculosis compared to those without the polymorphism.
Conclusion: The TNF-α-308 G/A gene variant is not associated with an increased risk of pulmonary
tuberculosis in the Cirebon community.
Keywords: TNF-α -308 G/A; Gene polymorphisms; Pulmonary tuberculosis; Tumor necrosis factor-alpha