Latar Belakang: Escherichia coli merupakan bakteri penyebab diare. Banyak yang melaporkan terjadinya resistensi antibiotik terhadap Escherichia coli. Beberapa penelitian mengatakan Daun Kemangi mengandung zat metabolit sekunder seperti alkaloid, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri.

Tujuan: Mengetahui Daya Hambat Fraksi Etil Asetat Daun Kemangi (Ocimum basilicum L) Terhadap Pertumbuhan Escherichia coli.

Metode: Penelitian ini adalah penelitian eksperimental laboratorium dengan rancangan penelitian post-test only control grup design. Penelitian ini menggunakan 5 kelompok, 2 kelompok kontrol dan 3 kelompok perlakuan. Kelompok perlakuan terdiri dari fraksi etil asetat dari daun kemangi konsentrasi 12,5%�, 25% �, 50% �. Kelompok kontrol yaitu kontrol positif (K(+)) dengan cotrimoxazole dan kontrol negatif (K(-)) yaitu Dimetil Sulfoksida (DMSO) 10%. Data diuji menggunakan uji Kruskal walls dan dilanjutkan dengan uji beda Mann Whitney

Hasil: Pada uji Kruskal walls terdapat perbedaan yang signifikan (p-value < 0,050), terhadap pemberian perlakuan fraksi etil asetat daun kemangi. Didapat rerata terbesar yaitu pada fraksi etil asetat konsentrasi 12,5%. Dilanjutkan dengan uji Mann whitney untuk mengetahui perbedaan antar kelompok, didapatkan perbedaan daya hambat pada masing-masing konsentrasi

Kesimpulan: Kadar hambat minimum dari fraksi etil asetat daun kemangi terhadap pertumbuhan Escherichia coli konsentrasi 12,5%, dan terdapat perbedaan yang bermakna setiap konsentrasi fraksi etil asetat daun kemangi terhadap pertumbuhan Escherichia coli

Kata Kunci: Daun Kemangi, Fraksi, Escherichia coli

Background: Escherichia coli is a bacterium that causes diarrhea. Many reported the occurrence of antibiotic resistance against Escherichia coli. Some studies say that basil leaves contain secondary metabolites such as alkaloids, flavonoids, saponins, and tannins that function as antibacterial.

Aim : Knowing the Inhibitory Power of the Ethyl Acetate Fraction of Basil Leaves (Ocimum basilicum L) on the growth of Escherichia coli.

Methods : This research is a laboratory experimental study with a post-test-only control group design. This study used 5 groups, 2 control groups and 3 treatment groups. The treatment group consisted of the ethyl acetate fraction of basil leaves at concentrations of 12.5% �, 25% �, and 50% �. The control group was a positive control K(+) with cotrimoxazole and negative control K(-) with 10% Dimethyl Sulfoxide (DMSO). The data were tested using the Kruskal walls test and continued with the Mann-Whitney difference test

Results : In the Kruskal Walls test, there was a significant difference (p-value < 0.050), for the treatment of basil leaf ethyl acetate fraction. The largest average was obtained, namely the ethyl acetate fraction with a concentration of 12.5%. Followed by the Mann-Whitney test to determine the differences between groups, there were differences in the inhibition power of each concentration.

Conclusions : The Minimum Inhibitory Level of the ethyl acetate fraction of basil leaves on the growth of Escherichia coli is a concentration of 12.5. Also, there is a significant difference in each concentration of the ethyl acetate fraction of basil leaves on the growth of Escherichia coli bacteria.

Keywords : Basil Leaves, Fraction, Escherichia coli